Hooper_1987_Biochem.J_244_465

Reference

Title : Renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase C - Hooper_1987_Biochem.J_244_465
Author(s) : Hooper NM , Low MG , Turner AJ
Ref : Biochemical Journal , 244 :465 , 1987
Abstract :

Renal dipeptidase (dehydropeptidase-I, EC 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase C from Staphylococcus aureus and Bacillus thuringiensis and a phospholipase C preparation from Bacillus cereus to a similar extent as alkaline phosphatase. Endopeptidase-24.11 and aminopeptidase N were not released by this treatment. After treatment of the membrane fraction with the S. aureus phospholipase C the dipeptidase was converted from an amphipathic to a hydrophilic form, as deduced from phase-separation experiments in Triton X-114. It is concluded that renal dipeptidase is anchored to the microvillar membrane by covalently attached phosphatidylinositol.

PubMedSearch : Hooper_1987_Biochem.J_244_465
PubMedID: 2822007

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Citations formats

Hooper NM, Low MG, Turner AJ (1987)
Renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase C
Biochemical Journal 244 :465

Hooper NM, Low MG, Turner AJ (1987)
Biochemical Journal 244 :465