| Title : A new screening method based on yeast-expressed human dipeptidyl peptidase IV and discovery of novel inhibitors - Hu_2009_Biotechnol.Lett_31_979 |
| Author(s) : Hu CX , Huang H , Zhang L , Huang Y , Shen ZF , Cheng KD , Du GH , Zhu P |
| Ref : Biotechnol Lett , 31 :979 , 2009 |
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Abstract :
Dipeptidyl peptidase (DPP) IV inhibitors provide a new strategy for the treatment of type 2 diabetes. Human DPP-IV gene was cloned from differentiated Caco-2 cells and expressed in Pichia pastoris. The recombinant enzyme was used in a new system for screening of DPP-IV inhibitors. By high throughput screening, a novel compound (W5188) was identified from 75,000 compounds with an IC(50) of 6.5 microM. This method is highly reproducible and reliable for discovery of DPP-IV inhibitors as shown by Z' value of 0.73 and S/N ratio of 6.89. |
| PubMedSearch : Hu_2009_Biotechnol.Lett_31_979 |
| PubMedID: 19267232 |
Hu CX, Huang H, Zhang L, Huang Y, Shen ZF, Cheng KD, Du GH, Zhu P (2009)
A new screening method based on yeast-expressed human dipeptidyl peptidase IV and discovery of novel inhibitors
Biotechnol Lett
31 :979
Hu CX, Huang H, Zhang L, Huang Y, Shen ZF, Cheng KD, Du GH, Zhu P (2009)
Biotechnol Lett
31 :979