| Title : Development of a sensitive method for quantitation of ABT-089 in plasma using fluorescence labeling with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole - Hui_1997_J.Chromatogr.B.Biomed.Sci.Appl_695_337 |
| Author(s) : Hui YH , Carroll S , Marsh KC |
| Ref : Journal of Chromatography B Biomed Sci Appl , 695 :337 , 1997 |
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Abstract :
A high-performance liquid chromatography method for the quantitation of ABT-089 [2-methyl-3-(2-(S)-pyrrolidinylmethoxy)pyridine] (I), a new structural type of cholinergic channel modulators (ChCM), is described in this paper using 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) as a fluorescent-labeling reagent. The method combined an optimized liquid-liquid extraction from plasma followed by pre-column derivatization to yield a fluorescence product. The selectivity, sensitivity, and reproducibility of this method were found to be excellent. This method was applied to the determination of ng/ml plasma and tissue levels of ABT-089 and similar compounds in biological samples. |
| PubMedSearch : Hui_1997_J.Chromatogr.B.Biomed.Sci.Appl_695_337 |
| PubMedID: 9300870 |
Hui YH, Carroll S, Marsh KC (1997)
Development of a sensitive method for quantitation of ABT-089 in plasma using fluorescence labeling with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole
Journal of Chromatography B Biomed Sci Appl
695 :337
Hui YH, Carroll S, Marsh KC (1997)
Journal of Chromatography B Biomed Sci Appl
695 :337