Hulme_1981_Eur.J.Pharmacol_73_137

Reference

Title : Two populations of binding sites for muscarinic antagonists in the rat heart - Hulme_1981_Eur.J.Pharmacol_73_137
Author(s) : Hulme EC , Berrie CP , Birdsall NJ , Burgen AS
Ref : European Journal of Pharmacology , 73 :137 , 1981
Abstract :

In a medium containing 100 mM NaCl and 10 mM MgCl2, the binding of antagonists to rat myocardial muscarinic receptors is well described by the simple Langmuir isotherm. However, in low ionic strength media, while a small proportion of the sites (23%) display a small (2-3 fold) increase in antagonist affinity, the majority demonstrate a substantial (ca. 10-fold) decrease. This reduction is reversed by micromolar concentrations of guanylylimidodiphosphate and other GTP analogues, with consequent abolition of the heterogeneity of the receptor population induced by low ionic strength. Millimolar concentrations of Mg2+ but not other cations strongly potentiate this action of the guanine nucleotides.

PubMedSearch : Hulme_1981_Eur.J.Pharmacol_73_137
PubMedID: 7308290

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Citations formats

Hulme EC, Berrie CP, Birdsall NJ, Burgen AS (1981)
Two populations of binding sites for muscarinic antagonists in the rat heart
European Journal of Pharmacology 73 :137

Hulme EC, Berrie CP, Birdsall NJ, Burgen AS (1981)
European Journal of Pharmacology 73 :137