Hwang_2022_Biochem.Biophys.Res.Commun_629_159

Reference

Title : Structural basis for the substrate specificity of an S-formylglutathione hydrolase derived from Variovorax sp. PAMC 28711 - Hwang_2022_Biochem.Biophys.Res.Commun_629_159
Author(s) : Hwang J , Kim B , Lee MJ , Nam Y , Youn UJ , Lee CS , Oh TJ , Park HH , Do H , Lee JH
Ref : Biochemical & Biophysical Research Communications , 629 :159 , 2022
Abstract :

S-Formylglutathione hydrolase was originally known to catalyze the hydrolysis of S-formylglutathione to formate and glutathione. However, this enzyme has a broader esterase activity toward substrates containing thioester and ester bonds. In a previous study, we identified a new S-formylglutathione hydrolase (VaSFGH) gene in the Antarctic bacterium Variovorax sp. PAMC 28711, and recombinant VaSFGH protein was purified and characterized. Previous enzyme activity assays showed that VaSFGH has high activity, especially toward short-chain p-nitrophenyl esters (C2-C4). In this study, we determined the crystal structure of substrate-free VaSFGH at a resolution of 2.38 A. In addition, p-nitrophenyl ester-bound VaSFGH structure models were generated by molecular docking simulations to obtain structural evidence of its substrate specificity. Comparative structural analysis of the apo-form and p-nitrophenyl ester-bound VaSFGH model structures revealed that large substrates could not bind inside the hydrophobic substrate-binding pocket because of the intrinsically static and relatively small substrate-binding pocket size of VaSFGH. This study provides useful information for further protein engineering of SFGHs for industrial use.

PubMedSearch : Hwang_2022_Biochem.Biophys.Res.Commun_629_159
PubMedID: 36122453
Gene_locus related to this paper: 9burk-a0a126zfj3

Related information

Gene_locus 9burk-a0a126zfj3
Structure 7YVT

Citations formats

Hwang J, Kim B, Lee MJ, Nam Y, Youn UJ, Lee CS, Oh TJ, Park HH, Do H, Lee JH (2022)
Structural basis for the substrate specificity of an S-formylglutathione hydrolase derived from Variovorax sp. PAMC 28711
Biochemical & Biophysical Research Communications 629 :159

Hwang J, Kim B, Lee MJ, Nam Y, Youn UJ, Lee CS, Oh TJ, Park HH, Do H, Lee JH (2022)
Biochemical & Biophysical Research Communications 629 :159