| Title : [Purification of acetylcholinesterase from human erythrocytes using metal-chelate affinity chromatography]. [Russian] - Il'ina_1996_Prikl.Biokhim.Mikrobiol_32_389 |
| Author(s) : Il'ina AV , Bannikova GE , Varlamov VP , Agabekian RS |
| Ref : Prikladnaia Biokhimiia i Mikrobiologiia , 32 :389 , 1996 |
|
Abstract :
Acetylcholinesterase (AChE) was purified on columns with iminodiacetate Agarose charged with Cu2+, Zn2+, and Ni2+. The best results (a 14-fold purification and more than 30% activity yield) were obtained with Zn2+ used as a complex-forming metal. The preparation had a specific activity of approximately 7 U. Its purity was tested by polyacrylamide gel electrophoresis under denaturing conditions. |
| PubMedSearch : Il'ina_1996_Prikl.Biokhim.Mikrobiol_32_389 |
| PubMedID: 8984476 |
Il'ina AV, Bannikova GE, Varlamov VP, Agabekian RS (1996)
[Purification of acetylcholinesterase from human erythrocytes using metal-chelate affinity chromatography]. [Russian]
Prikladnaia Biokhimiia i Mikrobiologiia
32 :389
Il'ina AV, Bannikova GE, Varlamov VP, Agabekian RS (1996)
Prikladnaia Biokhimiia i Mikrobiologiia
32 :389