Ilesanmi_2021_J.Ethnopharmacol__114592

Reference

Title : Neuroprotective flavonoids of the leaf of Antiaris africana Englea against cyanide toxicity - Ilesanmi_2021_J.Ethnopharmacol__114592
Author(s) : Ilesanmi OB , Akinmoladun AC , Elusiyan CA , Ogungbe IV , Olugbade TA , Olaleye TM
Ref : J Ethnopharmacol , :114592 , 2021
Abstract :

ETHNOPHARMACOLOGICAL RELEVANCE: Different parts of Antiaris africana Englea (Moraceae) are used traditionally for the treatment of various diseases, including epilepsy and nervous system disorders. AIMS OF THIS STUDY: The current study was designed to evaluate the neuroprotective activity of flavonoids isolated from A. africana against potassium cyanide (KCN)-induced oxidative damage in brain homogenate. MATERIALS AND METHODS: Dried and ground leaves of A. africana were extracted with methanol and fractioned into n-hexane (HFA), dichloromethane (DFA), ethyl acetate (EFA) and methanol (MFA). Each fraction was assessed for neuroprotective potential by anticholinesterase activity test. The fraction with the best anticholinesterase activity was subjected to various chromatographic techniques through bioassay-guided fractionation to isolate the bioactive compounds. The protective ability of the extract, fractions and compounds against Potassium cyanide (KCN)-induced mitochondrial damage in rat brain homogenate was evaluated. Structures of the isolated compounds were determined using 1D and 2D NMR, mass spectrometry and by comparison with literature data. RESULTS: and discussion: Ethyl acetate fraction showed the best anticholinesterase activity with an IC(50) of 23.23 +/- 1.12 microg/ml. Quercetin and a biflavonoid glucoside identified as 3'-4'-bisquercetin-3beta-D-diglucoside from this fraction were strongly antioxidant against DPPH and showed significant (P < 0.05) increase in the activity of dehydrogenase inhibited by KCN in a concentration dependent manner. However, quercetin showed more potency by reducing the MDA level and acetylcholinesterase activity elevated by KCN. CONCLUSION: Quercetin and the bisquercetin-diglucoside isolated from the leaves of A. Africana for the first time, are the major contributors to the observed neuroprotective property of the plant which supports its folkloric usage in the management of neurological disorder, seizures and epilepsy.

PubMedSearch : Ilesanmi_2021_J.Ethnopharmacol__114592
PubMedID: 34480996

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Citations formats

Ilesanmi OB, Akinmoladun AC, Elusiyan CA, Ogungbe IV, Olugbade TA, Olaleye TM (2021)
Neuroprotective flavonoids of the leaf of Antiaris africana Englea against cyanide toxicity
J Ethnopharmacol :114592

Ilesanmi OB, Akinmoladun AC, Elusiyan CA, Ogungbe IV, Olugbade TA, Olaleye TM (2021)
J Ethnopharmacol :114592