| Title : The application of HPLC with on-line coupled UV\/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from narcissus 'Sir Winston Churchill' - Ingkaninan_2000_J.Nat.Prod_63_803 |
| Author(s) : Ingkaninan K , Hazekamp A , de Best CM , Irth H , Tjaden UR , van der Heijden R , van der Greef J , Verpoorte R |
| Ref : Journal of Natural Products , 63 :803 , 2000 |
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Abstract :
An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE. |
| PubMedSearch : Ingkaninan_2000_J.Nat.Prod_63_803 |
| PubMedID: 10869205 |
Ingkaninan K, Hazekamp A, de Best CM, Irth H, Tjaden UR, van der Heijden R, van der Greef J, Verpoorte R (2000)
The application of HPLC with on-line coupled UV\/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from narcissus 'Sir Winston Churchill'
Journal of Natural Products
63 :803
Ingkaninan K, Hazekamp A, de Best CM, Irth H, Tjaden UR, van der Heijden R, van der Greef J, Verpoorte R (2000)
Journal of Natural Products
63 :803