Ishida_2002_Insect.Biochem.Mol.Biol_32_1775

Reference

Title : Cloning of putative odorant-degrading enzyme and integumental esterase cDNAs from the wild silkmoth, Antheraea polyphemus - Ishida_2002_Insect.Biochem.Mol.Biol_32_1775
Author(s) : Ishida Y , Leal WS
Ref : Insect Biochemistry & Molecular Biology , 32 :1775 , 2002
Abstract : Odorant-degrading enzymes have been postulated to participate in the fast deactivation of insect pheromones. These proteins are expressed specifically in the sensillar lymph of insect antennae in such low amounts that, hitherto, isolation and protein-based cDNA cloning has not been possible. Using degenerate primers based on conserved amino acid sequences of insect carboxylesterases and juvenile hormone esterases, we were able to amplify partial cDNA fragments, which were then used for the design of gene-specific primers for RACE. This bioinformatics approach led us to the cloning of cDNAs, encoding a putative odorant-degrading enzyme (Apol-ODE) and a putative integumental esterase (Apol-IE) from the wild silkmoth, Antheraea polyphemus. Apol-ODE had a predicted molecular mass of 59,994 Da, pI of 6.63, three potential N-glycosylation sites, and a putative catalytic site Ser characterized by the sequence Gly(195)-Glu-Ser-Ala-Gly-Ala. Apol-IE gave calculated molecular mass of 61,694 Da, pI of 7.49, two potential N-glycosylation sites, and a putative active site with the sequence Gly(214)-Tyr-Ser-Ala-Gly. The transcript of Apol-ODE was detected by RT-PCR in male antennae and branches (sensillar tissues), but not in female antennae and other control tissues. Apol-IE was detected in male and female antennae as well as legs.
ESTHER : Ishida_2002_Insect.Biochem.Mol.Biol_32_1775
PubMedSearch : Ishida_2002_Insect.Biochem.Mol.Biol_32_1775
PubMedID: 12429129
Gene_locus related to this paper: antpo-odest , antpo-tegest

Related information

Gene_locus related to this paper: antpo-odest , antpo-tegest

Citations formats

Ishida Y, Leal WS (2002)
Cloning of putative odorant-degrading enzyme and integumental esterase cDNAs from the wild silkmoth, Antheraea polyphemus
Insect Biochemistry & Molecular Biology 32 :1775

Ishida Y, Leal WS (2002)
Insect Biochemistry & Molecular Biology 32 :1775