Title : Organophosphorus hydrolase (OpdB) of Lactobacillus brevis WCP902 from kimchi is able to degrade organophosphorus pesticides - Islam_2010_J.Agric.Food.Chem_58_5380 |
Author(s) : Islam SM , Math RK , Cho KM , Lim WJ , Hong SY , Kim JM , Yun MG , Cho JJ , Yun HD |
Ref : Journal of Agricultural and Food Chemistry , 58 :5380 , 2010 |
Abstract :
Lactobacillus brevis WCP902 that is capable of biodegrading chlorpyrifos was isolated from kimchi. The opdB gene cloned from this strain revealed 825 bp, encoding 274 aa, and an enzyme molecular weight of about 27 kDa. OpdB contains the same Gly-X-Ser-X-Gly motif found in most bacterial and eukaryotic esterase, lipase, and serine hydrolases, yet it is a novel member of the GDSVG family of esterolytic enzymes. Its conserved serine residue, Ser82, is significantly involved with enzyme activity that may have application for removing some pesticides. Optimum organophosphorus hydrolase (OpdB) activity appeared at pH 6.0 and 35 degrees C and during degradation of chlorpyrifos, coumaphos, diazinon, methylparathion, and parathion. |
PubMedSearch : Islam_2010_J.Agric.Food.Chem_58_5380 |
PubMedID: 20405842 |
Gene_locus related to this paper: lacba-q03u96 |
Gene_locus | lacba-q03u96 |
Islam SM, Math RK, Cho KM, Lim WJ, Hong SY, Kim JM, Yun MG, Cho JJ, Yun HD (2010)
Organophosphorus hydrolase (OpdB) of Lactobacillus brevis WCP902 from kimchi is able to degrade organophosphorus pesticides
Journal of Agricultural and Food Chemistry
58 :5380
Islam SM, Math RK, Cho KM, Lim WJ, Hong SY, Kim JM, Yun MG, Cho JJ, Yun HD (2010)
Journal of Agricultural and Food Chemistry
58 :5380