| Title : Primary structure determination of mono- and diacylglycerol lipase from Penicillium camembertii - Isobe_1993_FEBS.Lett_320_101 |
| Author(s) : Isobe K , Nokihara K |
| Ref : FEBS Letters , 320 :101 , 1993 |
|
Abstract :
The complete amino acid sequence of mono- and diacylglycerol lipase from Penicillium camembertii was determined. This lipase has a single polypeptide chain consisting of 276 amino acid residues with two disulfide linkages. The primary structure was revealed by sequencing the digests of the intact and S-pyridylethylated proteins by trypsin, endoproteinase Lys-C and V8 protease. The two-dimensional electrophoresis was also carried out to confirm the internal sequence. The catalytic triad of this lipase was Ser, Asp and His, and one potential N-glycosylation site was also revealed. |
| PubMedSearch : Isobe_1993_FEBS.Lett_320_101 |
| PubMedID: 8458423 |
| Gene_locus related to this paper: penca-mdgli |
| Gene_locus | penca-mdgli |
Isobe K, Nokihara K (1993)
Primary structure determination of mono- and diacylglycerol lipase from Penicillium camembertii
FEBS Letters
320 :101
Isobe K, Nokihara K (1993)
FEBS Letters
320 :101