| Title : Purification of a presynaptic membrane protein that mediates a calcium-dependent translocation of acetylcholine - Israel_1986_Proc.Natl.Acad.Sci.U.S.A_83_9226 |
| Author(s) : Israel M , Morel Ni , Lesbats B , Birman S , Manaranche R |
| Ref : Proc Natl Acad Sci U S A , 83 :9226 , 1986 |
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Abstract :
A protein, which we call "mediatophore," that mediates calcium-dependent release of acetylcholine from proteoliposomes has been purified from the presynaptic plasma membrane. About 250 micrograms of this material was obtained from 500 g of Torpedo marmorata electric organ. Precipitation of the protein and subsequent removal of associated lipids inactivated the protein, which then became water soluble; this permitted evaluation of its Stokes radius (52 A) and its sedimentation coefficient (9.8 +/- 0.75 S) and, hence, an approximate molecular mass of 210 +/- 16 kDa could be determined. PAGE analysis showed that the protein is made of 17-kDa subunits, not linked by disulfide bonds. When this material was observed by electron microscopy after negative staining, the apparently pentameric structures had an average diameter of about 7 nm. |
| PubMedSearch : Israel_1986_Proc.Natl.Acad.Sci.U.S.A_83_9226 |
| PubMedID: 3466183 |
Israel M, Morel Ni, Lesbats B, Birman S, Manaranche R (1986)
Purification of a presynaptic membrane protein that mediates a calcium-dependent translocation of acetylcholine
Proc Natl Acad Sci U S A
83 :9226
Israel M, Morel Ni, Lesbats B, Birman S, Manaranche R (1986)
Proc Natl Acad Sci U S A
83 :9226