Title : Characterization of the protocatechuic acid catabolic gene cluster from Streptomyces sp. strain 2065 - Iwagami_2000_Appl.Environ.Microbiol_66_1499 |
Author(s) : Iwagami SG , Yang K , Davies J |
Ref : Applied Environmental Microbiology , 66 :1499 , 2000 |
Abstract :
Protocatechuate 3,4-dioxygenase (EC 1.13.11.3) catalyzes the ring cleavage step in the catabolism of aromatic compounds through the protocatechuate branch of the beta-ketoadipate pathway. A protocatechuate 3,4-dioxygenase was purified from Streptomyces sp. strain 2065 grown in p-hydroxybenzoate, and the N-terminal sequences of the beta- and alpha-subunits were obtained. PCR amplification was used for the cloning of the corresponding genes, and DNA sequencing of the flanking regions showed that the pcaGH genes belonged to a 6. 5-kb protocatechuate catabolic gene cluster; at least seven genes in the order pcaIJFHGBL appear to be transcribed unidirectionally. Analysis of the cluster revealed the presence of a pcaL homologue which encodes a fused gamma-carboxymuconolactone decarboxylase/beta-ketoadipate enol-lactone hydrolase previously identified in the pca gene cluster from Rhodococcus opacus 1CP. The pcaIJ genes encoded proteins with a striking similarity to succinyl-coenzyme A (CoA):3-oxoacid CoA transferases of eukaryotes and contained an indel which is strikingly similar between high-G+C gram-positive bacteria and eukaryotes. |
PubMedSearch : Iwagami_2000_Appl.Environ.Microbiol_66_1499 |
PubMedID: 10742233 |
Gene_locus related to this paper: strsp-PCAL |
Inhibitor | 3,4-dihydroxybenzoate |
Gene_locus | strsp-PCAL |
Iwagami SG, Yang K, Davies J (2000)
Characterization of the protocatechuic acid catabolic gene cluster from Streptomyces sp. strain 2065
Applied Environmental Microbiology
66 :1499
Iwagami SG, Yang K, Davies J (2000)
Applied Environmental Microbiology
66 :1499