Jabloune_2020_Microbes.Environ_35_

Reference

Title : Enzymatic Degradation of p-Nitrophenyl Esters, Polyethylene Terephthalate, Cutin, and Suberin by Sub1, a Suberinase Encoded by the Plant Pathogen Streptomyces scabies - Jabloune_2020_Microbes.Environ_35_
Author(s) : Jabloune R , Khalil M , Ben Moussa IE , Simao-Beaunoir AM , Lerat S , Brzezinski R , Beaulieu C
Ref : Microbes Environ , 35 : , 2020
Abstract :

The genome of Streptomyces scabies, the predominant causal agent of potato common scab, encodes a potential cutinase, the protein Sub1, which was previously shown to be specifically induced in the presence of suberin. The sub1 gene was expressed in Escherichia coli and the recombinant protein Sub1 was purified and characterized. The enzyme was shown to be versatile because it hydrolyzes a number of natural and synthetic substrates. Sub1 hydrolyzed p-nitrophenyl esters, with the hydrolysis of those harboring short carbon chains being the most effective. The Vmax and Km values of Sub1 for p-nitrophenyl butyrate were 2.36 mol g(-1) min(-1) and 5.7 10(-4) M, respectively. Sub1 hydrolyzed the recalcitrant polymers cutin and suberin because the release of fatty acids from these substrates was observed following the incubation of the enzyme with these polymers. Furthermore, the hydrolyzing activity of the esterase Sub1 on the synthetic polymer polyethylene terephthalate (PET) was demonstrated by the release of terephthalic acid (TA). Sub1 activity on PET was markedly enhanced by the addition of Triton and was shown to be stable at 37 degrees C for at least 20 d.

PubMedSearch : Jabloune_2020_Microbes.Environ_35_
PubMedID: 32101840
Gene_locus related to this paper: strsw-c9zcr8

Related information

Substrate Suberin
Gene_locus Suberin    strsw-c9zcr8

Citations formats

Jabloune R, Khalil M, Ben Moussa IE, Simao-Beaunoir AM, Lerat S, Brzezinski R, Beaulieu C (2020)
Enzymatic Degradation of p-Nitrophenyl Esters, Polyethylene Terephthalate, Cutin, and Suberin by Sub1, a Suberinase Encoded by the Plant Pathogen Streptomyces scabies
Microbes Environ 35 :

Jabloune R, Khalil M, Ben Moussa IE, Simao-Beaunoir AM, Lerat S, Brzezinski R, Beaulieu C (2020)
Microbes Environ 35 :