| Title : CRISPR-Knockout of CSE Gene Improves Saccharification Efficiency by Reducing Lignin Content in Hybrid Poplar - Jang_2021_Int.J.Mol.Sci_22_ |
| Author(s) : Jang HA , Bae EK , Kim MH , Park SJ , Choi NY , Pyo SW , Lee C , Jeong HY , Lee H , Choi YI , Ko JH |
| Ref : Int J Mol Sci , 22 : , 2021 |
| Abstract : Caffeoyl shikimate esterase (CSE) has been shown to play an important role in lignin biosynthesis in plants and is, therefore, a promising target for generating improved lignocellulosic biomass crops for sustainable biofuel production. Populus spp. has two CSE genes (CSE1 and CSE2) and, thus, the hybrid poplar (Populus alba x P. glandulosa) investigated in this study has four CSE genes. Here, we present transgenic hybrid poplars with knockouts of each CSE gene achieved by CRISPR/Cas9. To knockout the CSE genes of the hybrid poplar, we designed three single guide RNAs (sg1-sg3), and produced three different transgenic poplars with either CSE1 (CSE1-sg2), CSE2 (CSE2-sg3), or both genes (CSE1/2-sg1) mutated. CSE1-sg2 and CSE2-sg3 poplars showed up to 29.1% reduction in lignin deposition with irregularly shaped xylem vessels. However, CSE1-sg2 and CSE2-sg3 poplars were morphologically indistinguishable from WT and showed no significant differences in growth in a long-term living modified organism (LMO) field-test covering four seasons. Gene expression analysis revealed that many lignin biosynthetic genes were downregulated in CSE1-sg2 and CSE2-sg3 poplars. Indeed, the CSE1-sg2 and CSE2-sg3 poplars had up to 25% higher saccharification efficiency than the WT control. Our results demonstrate that precise editing of CSE by CRISPR/Cas9 technology can improve lignocellulosic biomass without a growth penalty. |
| ESTHER : Jang_2021_Int.J.Mol.Sci_22_ |
| PubMedSearch : Jang_2021_Int.J.Mol.Sci_22_ |
| PubMedID: 34575913 |
| Gene_locus related to this paper: arath-F14G24.3 |
| Gene_locus related to this paper: arath-F14G24.3 |
Jang HA, Bae EK, Kim MH, Park SJ, Choi NY, Pyo SW, Lee C, Jeong HY, Lee H, Choi YI, Ko JH (2021)
CRISPR-Knockout of CSE Gene Improves Saccharification Efficiency by Reducing Lignin Content in Hybrid Poplar
Int J Mol Sci
22 :
Jang HA, Bae EK, Kim MH, Park SJ, Choi NY, Pyo SW, Lee C, Jeong HY, Lee H, Choi YI, Ko JH (2021)
Int J Mol Sci
22 :