Jeon_2014_Appl.Microbiol.Biotechnol_98_5461

Reference

Title : Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha - Jeon_2014_Appl.Microbiol.Biotechnol_98_5461
Author(s) : Jeon JM , Brigham CJ , Kim YH , Kim HJ , Yi DH , Kim H , Rha C , Sinskey AJ , Yang YH
Ref : Applied Microbiology & Biotechnology , 98 :5461 , 2014
Abstract :

Polyhydroxyalkanoates (PHAs), a promising family of bio-based polymers, are considered to be alternatives to traditional petroleum-based plastics. Copolymers like poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) have been shown to exhibit favorable physical and mechanical properties, due to decreased crystallinity resulting from the presence of medium-chain-length 3-hydroxyhexanoate (3HHx) monomers. In this study, we produced P(HB-co-HHx) using engineered Ralstonia eutropha strains containing deletions of the acetoacetyl-CoA reductase (phaB) genes and replacing the native PHA synthase with phaC2 from Rhodococcus aetherivorans I24 and by using butyrate, a short-chain organic acid, as the carbon source. Although the wild-type R. eutropha did not produce P(HB-co-HHx) when grown on mixed acids or on butyrate as the sole carbon source, we are able to produce polymer containing up to 40 wt% 3HHx monomer with the aforementioned engineered R. eutropha strains using various concentrations of just butyrate as the sole carbon source. This is the first report for the production of P(HB-co-HHx) copolymer in R. eutropha using butyrate.

PubMedSearch : Jeon_2014_Appl.Microbiol.Biotechnol_98_5461
PubMedID: 24615385

Related information

Substrate P(3HB-co-3HHx)

Citations formats

Jeon JM, Brigham CJ, Kim YH, Kim HJ, Yi DH, Kim H, Rha C, Sinskey AJ, Yang YH (2014)
Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha
Applied Microbiology & Biotechnology 98 :5461

Jeon JM, Brigham CJ, Kim YH, Kim HJ, Yi DH, Kim H, Rha C, Sinskey AJ, Yang YH (2014)
Applied Microbiology & Biotechnology 98 :5461