Jiang_2009_Biochem.Biophys.Res.Commun_378_269

Reference

Title : Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae) - Jiang_2009_Biochem.Biophys.Res.Commun_378_269
Author(s) : Jiang X , Qu M , Denholm I , Fang J , Jiang W , Han Z
Ref : Biochemical & Biophysical Research Communications , 378 :269 , 2009
Abstract :

Two full-length genes encoding different acetylcholinesterases (AChEs), designated as Ch-ace1 and Ch-ace2, were cloned from strains of the rice stem borer (Chilo suppressalis) susceptible and resistant to the organophosphate insecticide triazophos. Sequence analysis found an amino acid mutation A314S in Ch-ace1 (corresponding to A201 in Torpedo californica AChE) that was consistently associated with the occurrence of resistance. This mutation removed an MspA1 I restriction site from the wild type allele. An assay based on restriction fragment length polymorphism (RFLP) analysis was developed to diagnose A314S genotypes in field populations. Results showed a strong correlation between frequencies of the mutation and phenotypic levels of resistance to triazophos. The assay offers a prospect for rapid monitoring of resistance and assisting with the appropriate choice of insecticide for combating damage caused by C. suppressalis.

PubMedSearch : Jiang_2009_Biochem.Biophys.Res.Commun_378_269
PubMedID: 19028456
Gene_locus related to this paper: 9neop-ACHE1 , 9neop-ACHE2

Related information

Citations formats

Jiang X, Qu M, Denholm I, Fang J, Jiang W, Han Z (2009)
Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae)
Biochemical & Biophysical Research Communications 378 :269

Jiang X, Qu M, Denholm I, Fang J, Jiang W, Han Z (2009)
Biochemical & Biophysical Research Communications 378 :269