Jiang_2025_Food.Chem_473_143090

Organophosphorus pesticides (OPs) are extensively utilized in agricultural production, but they pose significant threats to environment and organisms. This study aims to develop a novel method for the rapid and efficient detection of OPs. Initially, an iron/manganese zeolitic imidazolate framework (Fe/Mn-ZIF) with excellent oxidase-like activity was synthesised. The catalytic performance was evaluated, and the main factors influencing catalytic activity were investigated. Subsequently, a combined acetylcholinesterase assay was employed to detect three OPs. The specificity and recyclability of Fe/Mn-ZIF were also studied. The proposed colorimetric strategy demonstrated strong linear relationships: 0.1-2 mg/L for trichlorfon, 0.2-14 mg/L for glyphosate, and 0.4-10 mg/L for glufosinate, with the low detection limits of 0.024, 0.080, and 0.121 mg/L (3 S/N) respectively. Good recoveries were observed in real sample detection. This work lays a foundation for enhancing the catalytic performance of Fe/Mn-ZIF, which holds promise for biosensing applications.