Title : Overexpression and characterization of a new organic solvent-tolerant esterase derived from soil metagenomic DNA - Jin_2012_Bioresour.Technol_116_234 |
Author(s) : Jin P , Pei X , Du P , Yin X , Xiong X , Wu H , Zhou X , Wang Q |
Ref : Bioresour Technol , 116 :234 , 2012 |
Abstract :
In this study, an esterase, designated EstC23, was isolated from a soil metagenomic library. The protein was amenable to overexpression in Escherichia coli under control of the T7 promoter, resulting in expression of the active, soluble protein that constituted 30% of the total cell protein content. This enzyme showed optimal activity at 40 degrees C and retained about 50% maximal activity at 5-10 degrees C. EstC23 showed remarkable stability in up to 50% (v/v) benzene and alkanes (high logP solvents). When incubated for 7 days in the presence of 50% benzene or alkanes, the enzyme maintained its 2-3 fold elevated activity. The purified enzyme also cleaved sterically hindered esters of tertiary alcohols. These results indicate that EstC23 has potential for use in industrial processes. |
PubMedSearch : Jin_2012_Bioresour.Technol_116_234 |
PubMedID: 22100232 |
Gene_locus related to this paper: 9bact-q6rjm3 |
Gene_locus | 9bact-q6rjm3 |
Jin P, Pei X, Du P, Yin X, Xiong X, Wu H, Zhou X, Wang Q (2012)
Overexpression and characterization of a new organic solvent-tolerant esterase derived from soil metagenomic DNA
Bioresour Technol
116 :234
Jin P, Pei X, Du P, Yin X, Xiong X, Wu H, Zhou X, Wang Q (2012)
Bioresour Technol
116 :234