Johns_2015_J.Mol.Microbiol.Biotechnol_25_412

Reference

Title : The Escherichia coli yjfP Gene Encodes a Carboxylesterase Involved in Sugar Utilization during Diauxie - Johns_2015_J.Mol.Microbiol.Biotechnol_25_412
Author(s) : Johns N , Wrench A , Loto F , Valladares R , Lorca G , Gonzalez CF
Ref : J Molecular Microbiology Biotechnol , 25 :412 , 2015
Abstract :

BACKGROUND: Acetylation and efflux of carbohydrates during cellular metabolism is a well-described phenomenon associated with a detoxification process to prevent metabolic congestion. It is still unclear why cells discard important metabolizable energy sources in the form of acetylated compounds. METHODS: We describe the purification and characterization of an approximately 28-kDa intracellular carboxylesterase (YjfP) and the analysis of gene and protein expression by qRT-PCR and Western blot. RESULTS: qRT-PCR and Western blot, respectively, showed that yjfP is upregulated during the diauxic lag in cells growing with a mixture of glucose and lactose. The beta-galactosidase activity in the deltayjfP strain was both delayed and half the magnitude of that of the wild-type strain. YjfP-hyperproducing strains displayed a long lag phase when cultured with glucose and then challenged to grow with lactose or galactose as the sole carbon source. CONCLUSION: Our results suggest that YjfP controls the intracellular concentration of acetyl sugars by redirecting them to the main metabolic circuits. Instead of detoxification, we propose that sugar acetylation is utilized by the cell for protection and to prevent the metabolism of a necessary minimal intracellular sugar pool. Those sugars can eventually be exported as a side effect of these mechanisms.

PubMedSearch : Johns_2015_J.Mol.Microbiol.Biotechnol_25_412
PubMedID: 26820469
Gene_locus related to this paper: ecoli-yjfp

Related information

Gene_locus ecoli-yjfp
Family yjfP_esterase-like

Citations formats

Johns N, Wrench A, Loto F, Valladares R, Lorca G, Gonzalez CF (2015)
The Escherichia coli yjfP Gene Encodes a Carboxylesterase Involved in Sugar Utilization during Diauxie
J Molecular Microbiology Biotechnol 25 :412

Johns N, Wrench A, Loto F, Valladares R, Lorca G, Gonzalez CF (2015)
J Molecular Microbiology Biotechnol 25 :412