| Title : Metabolism of T-2 toxin by rat liver carboxylesterase - Johnsen_1986_Biochem.Pharmacol_35_1469 |
| Author(s) : Johnsen H , Odden E , Lie O , Johnsen BA , Fonnum F |
| Ref : Biochemical Pharmacology , 35 :1469 , 1986 |
| Abstract : The trichothecene T-2 toxin was rapidly hydrolyzed by rat liver microsomal fraction into HT-2 toxin which was the main metabolite. The metabolism was completely blocked by paraoxon, a serine esterase inhibitor, but not affected by EDTA or 4-hydroxy mercury benzoate, inhibitors of arylesterase and esterases containing SH-group in active site, respectively. Among the serine esterases carboxylesterase (EC 3.1.1.1), but not cholinesterase (EC 3.1.1.8) hydrolysed T-2 toxin to HT-2 toxin. Carboxylesterase activity from liver microsomes was separated into at least five different isoenzymes by isoelectric focusing, and only the isoenzyme of pI 5.4 was able to hydrolyse T-2 toxin to HT-2 toxin. The toxicity of T-2 toxin in mice was enhanced by pre-treatment with tri-o-cresyl phosphate (TOCP), a specific carboxylesterase inhibitor. This confirms the importance of carboxylesterase in detoxification of trichothecenes. |
| ESTHER : Johnsen_1986_Biochem.Pharmacol_35_1469 |
| PubMedSearch : Johnsen_1986_Biochem.Pharmacol_35_1469 |
| PubMedID: 3707611 |
Johnsen H, Odden E, Lie O, Johnsen BA, Fonnum F (1986)
Metabolism of T-2 toxin by rat liver carboxylesterase
Biochemical Pharmacology
35 :1469
Johnsen H, Odden E, Lie O, Johnsen BA, Fonnum F (1986)
Biochemical Pharmacology
35 :1469