Johnson_2000_Mol.Plant.Microbe.Interact_13_742

Reference

Title : Cloning and characterization of a cyclic peptide synthetase gene from Alternaria alternata apple pathotype whose product is involved in AM-toxin synthesis and pathogenicity - Johnson_2000_Mol.Plant.Microbe.Interact_13_742
Author(s) : Johnson RD , Johnson L , Itoh Y , Kodama M , Otani H , Kohmoto K
Ref : Mol Plant Microbe Interact , 13 :742 , 2000
Abstract :

Afternaria afternata apple pathotype causes Alternaria blotch of susceptible apple cultivars through the production of a cyclic peptide host-specific toxin, AM-toxin. PCR (polymerase chain reaction), with primers designed to conserved domains of peptide synthetase genes, amplified several products from A. alternata apple pathotype that showed high similarity to other fungal peptide synthetases and were specific to the apple pathotype. Screening of a Lambda Zap genomic library with these PCR-generated probes identified overlapping clones containing a complete cyclic peptide synthetase gene of 13.1 kb in length with no introns. Disruption of this gene, designated AM-toxin synthetase (AMT), by transformation of wild-type A. afternata apple pathotype with disruption vectors resulted in toxin-minus mutants, which were also unable to cause disease symptoms on susceptible apple cultivars. AM-toxin synthetase is therefore a primary determinant of virulence and specificity in the A. alternata apple pathotype/apple interaction.

PubMedSearch : Johnson_2000_Mol.Plant.Microbe.Interact_13_742
PubMedID: 10875335
Gene_locus related to this paper: altal-amt4

Related information

Gene_locus altal-amt4

Citations formats

Johnson RD, Johnson L, Itoh Y, Kodama M, Otani H, Kohmoto K (2000)
Cloning and characterization of a cyclic peptide synthetase gene from Alternaria alternata apple pathotype whose product is involved in AM-toxin synthesis and pathogenicity
Mol Plant Microbe Interact 13 :742

Johnson RD, Johnson L, Itoh Y, Kodama M, Otani H, Kohmoto K (2000)
Mol Plant Microbe Interact 13 :742