Jones_1996_Vet.Hum.Toxicol_38_1

Reference

Title : Method for screening drug and chemical effects in laboratory rats using computerized quantitative electroencephalography - Jones_1996_Vet.Hum.Toxicol_38_1
Author(s) : Jones RD , Sheets LP , Mueller RE
Ref : Vet Hum Toxicol , 38 :1 , 1996
Abstract :

A minimally-invasive method of quantitative electroencephalography (qEEG) that requires no anesthetics and parallels techniques of naturalistic stimulation was developed and validated for regulatory testing of drugs and chemicals in rats. Male and female Fischer 344 rats were utilized in a randomized-block design to measure qEEG target parameters associated with a range of cholinesterase inhibition. For this study, physostigmine was administered ip at doses of 0.05, 0.2 or 1.0 mg/kg, resulting in average cholinesterase inhibition in plasma (28, 38 and 70%), erythrocytes (19, 24 and 36%), and brain (2, 10 and 31%) which correlated well with increased total power and amplitude changes. Additional treatment-related effects consisted of decreased relative alpha and beta, increased relative delta, and a left-shift in the spectral-edge frequency. In a second study, male and female Sprague-Dawley rats were utilized in a treatment-by-subjects design to determine qEEG target parameter changes due to the M2 autoreceptor agonist oxotremorine. Repeated incremental doses (0.05, 0.1, 0.2 mg/kg; ip) of oxotremorine resulted in increased beta contribution, a right-shift in the spectral-edge frequency and decreased alpha contribution. These qEEG results with physostigmine and oxotremorine correlate well with receptor-specific and general muscarinic effects, making it a reliable contribution to analysis of agonist and antagonist effects of cholinergic compounds.

PubMedSearch : Jones_1996_Vet.Hum.Toxicol_38_1
PubMedID: 8825739

Related information

Citations formats

Jones RD, Sheets LP, Mueller RE (1996)
Method for screening drug and chemical effects in laboratory rats using computerized quantitative electroencephalography
Vet Hum Toxicol 38 :1

Jones RD, Sheets LP, Mueller RE (1996)
Vet Hum Toxicol 38 :1