| Title : Characterization and preparation of highly stable aggregates of a novel type of hydrolase (BL28) from Bacillus licheniformis - Ju_2013_Bioresour.Technol_128_81 |
| Author(s) : Ju H , Jang E , Ryu BH , Kim TD |
| Ref : Bioresour Technol , 128 :81 , 2013 |
|
Abstract :
A novel type of hydrolase (BL28) from Bacillus licheniformis was identified, expressed in Escherichia coli, characterized, and immobilized for industrial applications. Biochemical characteristics of BL28 were investigated by performing SDS-PAGE, mass spectrometry, enzyme assays, CD spectroscopy, intrinsic fluorescence, and in silico analysis. Furthermore, cross-linked enzyme aggregates (CLEAs) of BL28 were prepared. These CLEA-BL28 aggregates exhibited improved catalytic efficiencies and stabilities compared to free BL28 against harsh conditions of thermal or chemical stress as well as high reusability. The characteristics of the CLEA-BL28 aggregates highlight their great potentials in pharmaceutical and chemical industries. |
| PubMedSearch : Ju_2013_Bioresour.Technol_128_81 |
| PubMedID: 23196225 |
| Gene_locus related to this paper: bacld-q65eq1 |
| Gene_locus | bacld-q65eq1 |
Ju H, Jang E, Ryu BH, Kim TD (2013)
Characterization and preparation of highly stable aggregates of a novel type of hydrolase (BL28) from Bacillus licheniformis
Bioresour Technol
128 :81
Ju H, Jang E, Ryu BH, Kim TD (2013)
Bioresour Technol
128 :81