Title : Efficient Synthesis of Stearidonic Acid Enriched Triacylglycerol from Ahiflower Seed Oil via a Two-Step Enzyme Reaction - Ju_2022_J.Oleo.Sci_71_1679 |
Author(s) : Ju C , Lee YJ , Yoon HS , Kim BH , Kim IH |
Ref : J Oleo Sci , 71 :1679 , 2022 |
Abstract :
Stearidonic acid (SDA) is a plant-based n-3 polyunsaturated fatty acid with multiple biological activities. The enrichment of SDA and synthesis of triacylglycerol (TAG) were carried out consecutively via two lipase-catalyzed reactions, hydrolysis, and esterification. First, SDA was enriched into a glyceride fraction from ahiflower seed oil by Candida rugosa lipase-catalyzed hydrolysis. Under the optimum conditions of 35 degreesC, 0.1% lipase powder of Lipase OF, and 50% buffer solution (based on the weight of total substrate), SDA was enriched from 21.6 to 40.7 wt% in glyceride fraction. SDA-enriched TAG was then synthesized from the SDA-enriched glyceride and the SDA-enriched fatty acid via esterification using an in-house immobilized lipase as a biocatalyst. The SDA-enriched fatty acid was obtained from part of the SDA-enriched glyceride by saponification and the in-house immobilized lipase was prepared from Eversa((a)) Transform 2.0 using Lewatit VP OC 1600 as a carrier. The optimum reaction conditions for the synthesis of TAG were a temperature of 50 degreesC, an enzyme loading of 10%, and a vacuum of 10 mmHg. A maximum conversion to TAG of ca. 94% was achieved after 12 h under the optimum conditions. |
PubMedSearch : Ju_2022_J.Oleo.Sci_71_1679 |
PubMedID: 36310055 |
Ju C, Lee YJ, Yoon HS, Kim BH, Kim IH (2022)
Efficient Synthesis of Stearidonic Acid Enriched Triacylglycerol from Ahiflower Seed Oil via a Two-Step Enzyme Reaction
J Oleo Sci
71 :1679
Ju C, Lee YJ, Yoon HS, Kim BH, Kim IH (2022)
J Oleo Sci
71 :1679