Junot_2001_J.Immunoassay.Immunochem_22_15

Reference

Title : Development of an enzyme immunoassay for a stable amidated analog of the hemoregulatory peptide acetyl-Ser-Asp-Lys-Pro - Junot_2001_J.Immunoassay.Immunochem_22_15
Author(s) : Junot C , Theodoro F , Thierry J , Clement G , Wdzieczak-Bakala J , Ezan E
Ref : Journal of Immunoassay Immunochem , 22 :15 , 2001
Abstract :

The tetrapeptide Acetyl-Ser-Asp-Lys-Pro (AcSDKP) has been shown to protect hematopoietic stem cells from the toxicity of anticancer chemotherapies. Since its pharmacological efficacy is limited by a rapid degradation by Angiotensin-I Converting Enzyme (ACE), AcSDKP analogs resistant to ACE have been synthesized. One of these compounds (AcSDKP-NH,) differs from the native AcSDKP by amidation of the C-terminus. Further evaluations of this molecule require an analytical method in order to characterize its pharmacokinetic profile. We report, here, the development of a highly specific and sensitive enzyme immunoassay (EIA) for AcSDKP-NH, thatdoes not cross-react with endogenous or exogenous AcSDKP. Using AcSDKP-NH2-acetylcholinesterase conjugate as a tracer, rabbit specific antiserum and microtiter plates coated with goat anti-rabbit immunoglobulins, this EIA allows the determination of AcSDKP-NH2 with limits of quantitation of 1 nM in mouse plasma and 100 pmol/g in tissues. Intra-day and inter-day coefficients of variations were less than 20%. The method was successfully applied to a pharmacokinetic study in order to compare plasma and tissue profiles of AcSDKP-NH2 and AcSDKP. Plasma AcSDKP-NH2 levels were found higher than those of AcSDKP, with AUCinf and Cmax values, respectively, 26- and 10-fold higher than that of AcSDKP.

PubMedSearch : Junot_2001_J.Immunoassay.Immunochem_22_15
PubMedID: 11486817

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Citations formats

Junot C, Theodoro F, Thierry J, Clement G, Wdzieczak-Bakala J, Ezan E (2001)
Development of an enzyme immunoassay for a stable amidated analog of the hemoregulatory peptide acetyl-Ser-Asp-Lys-Pro
Journal of Immunoassay Immunochem 22 :15

Junot C, Theodoro F, Thierry J, Clement G, Wdzieczak-Bakala J, Ezan E (2001)
Journal of Immunoassay Immunochem 22 :15