Juntachai_2011_Microbiol.(Reading)_157_3492

Reference

Title : Purification and characterization of a secretory lipolytic enzyme, MgLIP2, from Malassezia globosa - Juntachai_2011_Microbiol.(Reading)_157_3492
Author(s) : Juntachai W , Oura T , Kajiwara S
Ref : Microbiology (Reading) , 157 :3492 , 2011
Abstract :

Malassezia globosa is a lipid-dependent yeast that is found on the human skin and is associated with various skin disorders, including dandruff and seborrhoeic dermatitis (SD). Despite its important role in skin diseases, the molecular basis for its pathogenicity is poorly understood. The current hypothesis is that dandruff and SD are linked to fatty acid metabolism and secretory lipolytic enzymes, which hydrolyse sebaceous lipids and release irritating free fatty acids. A previous genomic analysis of M. globosa identified a family of 13 homologous genes predicted to encode secreted lipases. We have also reported that M. globosa had significantly higher extracellular lipase activity compared with other species. To identify the major secretory lipases of this yeast during its growth, we successfully purified and characterized an extracellular lipase MgLIP2. Based on MALDI-TOF MS, the peptide mass fingerprint of a tryptically digested protein MgLIP2 corresponded to ORF MGL_4054 of M. globosa. This lipase showed high esterase activity against 4-nitrophenyl palmitate and 1-naphthyl palmitate but not 1-naphthyl acetate. This enzyme had optimal activity at 30 degreesC and pH 5.0. Furthermore, the activity significantly increased in the presence of Triton X-100 and was partially inhibited by PMSF but was unaffected by univalent and divalent metal ions.

PubMedSearch : Juntachai_2011_Microbiol.(Reading)_157_3492
PubMedID: 22016565
Gene_locus related to this paper: malgo-a8qcw4

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Citations formats

Juntachai W, Oura T, Kajiwara S (2011)
Purification and characterization of a secretory lipolytic enzyme, MgLIP2, from Malassezia globosa
Microbiology (Reading) 157 :3492

Juntachai W, Oura T, Kajiwara S (2011)
Microbiology (Reading) 157 :3492