| Title : Fatty acid composition, enzyme inhibitory effect, antioxidant and anticancer activity of extract from Saponaria prostrata WILLD. subsp. anatolica HEDGE - Kadir_2021_Bioorg.Chem_113_105032 |
| Author(s) : Kadir Erdogan M , Halil Gecibesler I , Yapar Y , Gundogdu R , Kirici M , Behcet L , Taslimi P |
| Ref : Bioorg Chem , 113 :105032 , 2021 |
| Abstract : This study attempts to evaluate the antioxidant, enzyme inhibitory, and anticancer properties as well as fatty acid compositions of endemic Saponaria prostrata WILLD. subsp. anatolica HEDGE. The gas chromatography-mass spectrometry (GC-MS) was used to determine the fatty acid content of methanol: dichloromethane extract from S. prostrata subsp. anatolica (SPA). Enzymatic activity was measured against acetylcholinesterase, butyrylcholinesterase and alpha-glucosidase. DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity and Ferric reducing antioxidant power assay (FRAP) were conducted to antioxidant properties. The anticancer effect of SPA on human MCF-7 breast cancer and human HCT116 colorectal cancer cell line was evaluated by WST-1 cell viability assay, colony formation assay and wound healing assay. In addition, human VEGF Elisa method was used to determine the anti-angiogenic effect, and the quantitative real-time PCR (qRT-PCR) method on p53, Bax and Bcl-2 mRNA levels were used to evaluate apoptosis. While high amounts of palmitic acid (40.8%), linoleic acid (17.75%) and alpha-linolenic acid (16.84%) were detected in the SPA, the total amount of unsaturated fatty acid (51.34%) was higher than the total amount of saturated fatty acid (48.66%). SPA displayed the most promising acetylcholinesterase (AChE), butyrylcholinesterase (BuChE) and alpha-glycosidase (AG) inhibitory activities (AChE: IC(50): 18.03 microg/mL, BuChE: IC(50): 44.24 microg/mL and AG: IC(50): 210.85 microg/mL). The half maximum inhibitory concentration (IC(50)) of SPA in MCF-7 and HCT116 cells was determined as 259.79 microg/mL and 97.24 microg/mL, respectively. In addition, it was determined that SPA suppresses colony formation and wound closure, and suppresses angiogenesis as well as triggering apoptosis at a significant level. It is true that endemic S. prostrata subsp. anatolica is a potential source of functional food ingredients, but more analytical and in vivo experiments are needed to explore further secondary metabolite diversity and pharmacological properties. |
| ESTHER : Kadir_2021_Bioorg.Chem_113_105032 |
| PubMedSearch : Kadir_2021_Bioorg.Chem_113_105032 |
| PubMedID: 34089947 |
Kadir Erdogan M, Halil Gecibesler I, Yapar Y, Gundogdu R, Kirici M, Behcet L, Taslimi P (2021)
Fatty acid composition, enzyme inhibitory effect, antioxidant and anticancer activity of extract from Saponaria prostrata WILLD. subsp. anatolica HEDGE
Bioorg Chem
113 :105032
Kadir Erdogan M, Halil Gecibesler I, Yapar Y, Gundogdu R, Kirici M, Behcet L, Taslimi P (2021)
Bioorg Chem
113 :105032