| Title : Acylation of soluble polysaccharides in a biphasic system catalyzed by a CE2 acetyl esterase - Kanelli_2017_Carbohydr.Polym_163_208 |
| Author(s) : Kanelli M , Topakas E |
| Ref : Carbohydr Polym , 163 :208 , 2017 |
| Abstract : Within the last decade, acylated polysaccharides have drawn attention, since they find applications in numerous fields as biocompatible and biodegradable amphiphilic compounds. The ability of a CE2 acetyl esterase from Clostridium thermocellum to catalyze acyl transfer to beta-glucan and manno-polysaccharides of significant interest was investigated. Initially, screening tests were conducted on aldohexose monosaccharides and disaccharides, exploiting the enzyme's strict regioselectivity at O-6 position. Modified monosaccharides acquired acylation yields from 11 up to 65%, showing preference for small chain acyl donors, while disaccharides exhibited conversion yields from 23 up to 58%, with preference to monoacylation. The transesterification reactions were carried out in two-phase mixtures consisted of water/vinyl esters. Acylation of polysaccharides were confirmed by TLC and FT-IR, while the degree of acylation was determined via an indirect method, estimating a range of acylation from 0.022 to 1.083mmolacylgroup*gpolysaccharide-1 depending on the structure and composition of the target polysaccharide. |
| ESTHER : Kanelli_2017_Carbohydr.Polym_163_208 |
| PubMedSearch : Kanelli_2017_Carbohydr.Polym_163_208 |
| PubMedID: 28267499 |
Kanelli M, Topakas E (2017)
Acylation of soluble polysaccharides in a biphasic system catalyzed by a CE2 acetyl esterase
Carbohydr Polym
163 :208
Kanelli M, Topakas E (2017)
Carbohydr Polym
163 :208