Kang_2015_Biochem.Biophys.Res.Commun_468_611

Reference

Title : Inhibition of osteoclast differentiation by overexpression of NDRG2 in monocytes - Kang_2015_Biochem.Biophys.Res.Commun_468_611
Author(s) : Kang K , Nam S , Kim B , Lim JH , Yang Y , Lee MS , Lim JS
Ref : Biochemical & Biophysical Research Communications , 468 :611 , 2015
Abstract :

N-Myc downstream-regulated gene 2 (NDRG2), a member of the NDRG family of differentiation-related genes, has been characterized as a regulator of dendritic cell differentiation from monocytes, CD34(+) progenitor cells, and myelomonocytic leukemic cells. In this study, we show that NDRG2 overexpression inhibits the differentiation of U937 cells into osteoclasts in response to stimulation with a combination of macrophage colony-stimulating factor (M-CSF) and soluble receptor activator of NF-kappaB ligand (RANKL). U937 cells stably expressing NDRG2 are unable to differentiate into multinucleated osteoclast-like cells and display reduced tartrate-resistant acid phosphatase (TRAP) activity and resorption pit formation. Furthermore, NDRG2 expression significantly suppresses the expression of genes that are crucial for the proliferation, survival, differentiation, and function of osteoclasts, including c-Fos, Atp6v0d2, RANK, and OSCAR. The activation of ERK1/2 and p38 is also inhibited by NDRG2 expression during osteoclastogenesis, and the inhibition of osteoclastogenesis by NDRG2 correlates with the down-regulation of the expression of the transcription factor PU.1. Taken together, our results suggest that the expression of NDRG2 potentially inhibits osteoclast differentiation and plays a role in modulating the signal transduction pathway responsible for osteoclastogenesis.

PubMedSearch : Kang_2015_Biochem.Biophys.Res.Commun_468_611
PubMedID: 26546825

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Citations formats

Kang K, Nam S, Kim B, Lim JH, Yang Y, Lee MS, Lim JS (2015)
Inhibition of osteoclast differentiation by overexpression of NDRG2 in monocytes
Biochemical & Biophysical Research Communications 468 :611

Kang K, Nam S, Kim B, Lim JH, Yang Y, Lee MS, Lim JS (2015)
Biochemical & Biophysical Research Communications 468 :611