Karunaratne_1993_Biochem.J_294 ( Pt 2)_575

Reference

Title : Characterization of a B-type esterase involved in insecticide resistance from the mosquito Culex quinquefasciatus - Karunaratne_1993_Biochem.J_294 ( Pt 2)_575
Author(s) : Karunaratne SH , Jayawardena KG , Hemingway J , Ketterman AJ
Ref : Biochemical Journal , 294 ( Pt 2) :575 , 1993
Abstract :

The enzyme, esterase B2, involved in insecticide resistance has been purified and characterized from the mosquito Culex quinquefasciatus. The monomeric enzyme has an M(r) of 62000 and a pI of 5.0. This enzyme is compared with the esterase A2 previously characterized [Ketterman, Jayawardena and Hemingway (1992) Biochem. J. 287, 355-360]. The kinetic constants for interaction with several insecticides indicate, as for the esterase A2, that the B2 enzyme has a role in resistance. The rates and affinities of binding observed support the hypothesis that the role mainly is sequestration followed by the slow turnover of the insecticide. Although the B2 esterase appears to have a slightly higher rate of interaction with insecticides, the A2 has a much greater Vmax. with the xenobiotic substrates studied. The B2 esterase also appears to be present in the larvae to a lesser extent than the esterase A2.

PubMedSearch : Karunaratne_1993_Biochem.J_294 ( Pt 2)_575
PubMedID: 8373372

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Citations formats

Karunaratne SH, Jayawardena KG, Hemingway J, Ketterman AJ (1993)
Characterization of a B-type esterase involved in insecticide resistance from the mosquito Culex quinquefasciatus
Biochemical Journal 294 ( Pt 2) :575

Karunaratne SH, Jayawardena KG, Hemingway J, Ketterman AJ (1993)
Biochemical Journal 294 ( Pt 2) :575