Kasrayan_2007_Chembiochem_8_1409

Reference

Title : Prediction of the Candida antarctica lipase A protein structure by comparative modeling and site-directed mutagenesis - Kasrayan_2007_Chembiochem_8_1409
Author(s) : Kasrayan A , Bocola M , Sandstrom AG , Laven G , Backvall JE
Ref : Chembiochem , 8 :1409 , 2007
Abstract :

A number of model structures of the CalA suggested by comparative modeling were tested by site-directed mutagenesis. Enzyme variants were created where amino acids predicted to play key roles for the lipase activity in the different models were replaced by an inert amino acid (alanine). The results from activity measurements of the overproduced and purified mutant enzymes indicate a structure where the active site consists of amino acid residues Ser184, His366, and Asp334 and in which there is no lid. This model can be used for future targeted modifications of the enzyme to obtain new substrate acceptance, better thermostability, and higher enantioselectivity.

PubMedSearch : Kasrayan_2007_Chembiochem_8_1409
PubMedID: 17631665

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Citations formats

Kasrayan A, Bocola M, Sandstrom AG, Laven G, Backvall JE (2007)
Prediction of the Candida antarctica lipase A protein structure by comparative modeling and site-directed mutagenesis
Chembiochem 8 :1409

Kasrayan A, Bocola M, Sandstrom AG, Laven G, Backvall JE (2007)
Chembiochem 8 :1409