| Title : Cloning and characterization of a gene cluster for hatomarubigin biosynthesis in Streptomyces sp. strain 2238-SVT4 - Kawasaki_2010_Appl.Environ.Microbiol_76_4201 |
| Author(s) : Kawasaki T , Hirashima R , Maruta T , Sato H , Maeda A , Yamada Y , Takeda M , Hayakawa Y |
| Ref : Applied Environmental Microbiology , 76 :4201 , 2010 |
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Abstract :
Streptomyces sp. strain 2238-SVT4 produces hatomarubigins A, B, C, and D, which belong to the angucycline family. Among them, hatomarubigin D has a unique dimeric structure with a methylene linkage. PCR using aromatase and cyclase gene-specific primers identified the hrb gene cluster for angucycline biosynthesis in Streptomyces sp. 2238-SVT4. The cluster consisted of 30 open reading frames, including those for the minimal polyketide synthase, ketoreductase, aromatase, cyclase, O-methyltransferase, oxidoreductase, and oxygenase genes. Expression of a part of the gene cluster containing hrbR1 to hrbX in Streptomyces lividans TK23 resulted in the production of hatomarubigins A, B, and C. Hatomarubigin D was obtained from the conversion of hatomarubigin C by a purified enzyme encoded by hrbY, among the remaining genes. |
| PubMedSearch : Kawasaki_2010_Appl.Environ.Microbiol_76_4201 |
| PubMedID: 20453135 |
Kawasaki T, Hirashima R, Maruta T, Sato H, Maeda A, Yamada Y, Takeda M, Hayakawa Y (2010)
Cloning and characterization of a gene cluster for hatomarubigin biosynthesis in Streptomyces sp. strain 2238-SVT4
Applied Environmental Microbiology
76 :4201
Kawasaki T, Hirashima R, Maruta T, Sato H, Maeda A, Yamada Y, Takeda M, Hayakawa Y (2010)
Applied Environmental Microbiology
76 :4201