Kempka_2008_Bioprocess.Biosyst.Eng_31_119

Reference

Title : Response surface method to optimize the production and characterization of lipase from Penicillium verrucosum in solid-state fermentation - Kempka_2008_Bioprocess.Biosyst.Eng_31_119
Author(s) : Kempka AP , Lipke NL , da Luz Fontoura Pinheiro T , Menoncin S , Treichel H , Freire DM , Di Luccio M , de Oliveira D
Ref : Bioprocess Biosyst Eng , 31 :119 , 2008
Abstract :

Current studies about lipase production by solid-state fermentation involve the use of agro-industrial residues towards developing cost-effective systems directed to large-scale commercialization of enzyme-catalyzed processes. In this work, lipase production and partial characterization of the crude enzymatic extracts obtained by Penicillium verrucosum using soybean bran as substrate was investigated. Different inductors were evaluated and the results showed that there is no influence of this variable on the lipase production, while temperature and initial moisture were the main factors that affected enzyme production. The optimized cultivation temperature (27.5 degrees C) and initial moisture of substrate (55%) were determined using the response surface methodology. Kinetics of lipase production was followed at the optimized growth conditions. Optimum lipase yield was 40 U/g of dry bran. The crude enzymatic extract showed optimal activity in the range from 30 to 45 degrees C and in pH 7.0.

PubMedSearch : Kempka_2008_Bioprocess.Biosyst.Eng_31_119
PubMedID: 17694331

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Citations formats

Kempka AP, Lipke NL, da Luz Fontoura Pinheiro T, Menoncin S, Treichel H, Freire DM, Di Luccio M, de Oliveira D (2008)
Response surface method to optimize the production and characterization of lipase from Penicillium verrucosum in solid-state fermentation
Bioprocess Biosyst Eng 31 :119

Kempka AP, Lipke NL, da Luz Fontoura Pinheiro T, Menoncin S, Treichel H, Freire DM, Di Luccio M, de Oliveira D (2008)
Bioprocess Biosyst Eng 31 :119