Khare_2019_Biomarkers__1

Synergy occurs when chemicals gives pronounced effect on combination in contrast to their individual effect. The objective of study was to investigate synergistic effect of pesticides Carbayl (C) and Methyl Parathion (MP) on oxidative stress biomarkers viz Catalase (CAT), Glutathione Reductase (GSSG-R) including different enzymes like Lactate Dehydrogenase (LDH), Succinate Dehydrogenase (SDH) and Acetyl cholinesterase (AChE) in different tissues of carps Catla Catla. Fishes were exposed to 6.25 mg/L of MP and 2.3 mg/L of C in mixture (1/3(rd) of LC50 Value). CAT and GSSG-R were studied in gills, brain, liver and muscle of carp were found to be elevated significantly (p< 0.005). LDH activity increased significantly (p< 0.005) in synergistic group, there was a 7 fold (748%) increase in LDH activity in muscle compared to individual studies with same pesticides. Contrary to LDH, sudden decrease in SDH activity was accounted. Significant (p< 0.005) decrease in AChE activity after initial 24hrs was remarkable addressing to the shift in neurotransmission pathway in organism. Significant increase was observed in activity of CAT and GSSG-R in all tissues compared to control fishes in individual as well as synergistic (MP + C) group suggesting that CAT and GSSG-R can be a potential biomarker of oxidative stress when studied in combination.