Khoo_2012_Funct.Plant.Biol_39_222

Reference

Title : Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics. - Khoo_2012_Funct.Plant.Biol_39_222
Author(s) : Khoo KHP , Able AJ , Chataway TK , Able JA
Ref : Funct Plant Biol , 39 :222 , 2012
Abstract :

Cereal coleoptile cell walls have exo- and endoglucanases capable of mediating the hydrolysis of non-cellulosic beta-(l,3)(l,4)-glucan in situ. A purified exoglucanase (EC 3.2.1.58) resolved as a single band at 73.5 kDa, while endoglucanase isozymes consistently appeared as two bands at 32.9 and 34.3 kDa when subjected to SDS-PAGE. HPLC analysis of the native proteins by gel-permeation chromatography revealed molecular weights of ca. 55 and 29 kDa for the exo- and endoglucanases, respectively. The exoglucanase has an isoelectric focusing point at pi 7.2 and the endoglucanase isozymes appeared as two major bands, one at pi 7.8 and another at 7.3. Deglycosylation of the native proteins followed by SDS-PAGE demonstrated that sugars accounted for ca. 6.5 % of the exoglucanase and were 12.5 and 8.8 % of the two endoglucanase isozymes, respectively. After deglycosylation the two endoglucanases converged at 30.0 kDa, suggesting polypeptide homology and that divergence in electrophoretic mobility was a consequence of glycosylation. Antibodies raised against intact exo- and endoglucanases recognized the polypeptide of the corresponding enzymes, irrespective of glycosylation. The N-terminal amino acid sequence supported the conclusion that the exo- and endoglucanase have different polypeptide structures.

PubMedSearch : Khoo_2012_Funct.Plant.Biol_39_222
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Gene_locus related to this paper: wheat-h8zi05

Related information

Gene_locus wheat-h8zi05

Citations formats

Khoo KHP, Able AJ, Chataway TK, Able JA (2012)
Preliminary characterisation of two early meiotic wheat proteins after identification through 2D gel electrophoresis proteomics.
Funct Plant Biol 39 :222

Khoo KHP, Able AJ, Chataway TK, Able JA (2012)
Funct Plant Biol 39 :222