| Title : A novel assay reveals the blockade of esterases by piperonyl butoxide - Khot_2008_Pest.Manag.Sci_64_1139 |
| Author(s) : Khot AC , Bingham G , Field LM , Moores GD |
| Ref : Pest Manag Sci , 64 :1139 , 2008 |
| Abstract : BACKGROUND: Conventional in vitro assays sometimes fail to reveal esterase inhibition by piperonyl butoxide (PBO), although synergism studies suggest loss of esterase-mediated sequestration of insecticide does take place. A new in vitro assay has been devised that routinely reveals binding between PBO and these esterases. RESULTS: The new 'esterase interference' assay detects the blockade of resistance-associated esterases in a species, Myzus persicae Slzer, where this has not previously been seen. The assay also demonstrates directly the protective effect esterases may confer to target sites of insecticides. CONCLUSION: The new assay reveals esterase blockade by PBO and thus has the potential to be used as a high-throughput screening method for other potential synergists. |
| ESTHER : Khot_2008_Pest.Manag.Sci_64_1139 |
| PubMedSearch : Khot_2008_Pest.Manag.Sci_64_1139 |
| PubMedID: 18481337 |
Khot AC, Bingham G, Field LM, Moores GD (2008)
A novel assay reveals the blockade of esterases by piperonyl butoxide
Pest Manag Sci
64 :1139
Khot AC, Bingham G, Field LM, Moores GD (2008)
Pest Manag Sci
64 :1139