Title : Presynaptic activation of silent synapses and growth of new synapses contribute to intermediate and long-term facilitation in Aplysia - Kim_2003_Neuron_40_151 |
Author(s) : Kim JH , Udo H , Li HL , Youn TY , Chen M , Kandel ER , Bailey CH |
Ref : Neuron , 40 :151 , 2003 |
Abstract :
The time course and functional significance of the structural changes associated with long-term facilitation of Aplysia sensory to motor neuron synaptic connections in culture were examined by time-lapse confocal imaging of individual sensory neuron varicosities labeled with three different fluorescent markers: the whole-cell marker Alexa-594 and two presynaptic marker proteins-synaptophysin-eGFP to monitor changes in synaptic vesicle distribution and synapto-PHluorin to monitor active transmitter release sites. Repeated pulses of serotonin induce two temporally, morphologically, and molecularly distinct presynaptic changes: (1) a rapid activation of silent presynaptic terminals by filling of preexisting empty varicosities with synaptic vesicles, which parallels intermediate-term facilitation, is completed within 3-6 hr and requires translation but not transcription and (2) a slower generation of new functional varicosities which occurs between 12-18 hr and requires transcription and translation. Enrichment of empty varicosities with synaptophysin accounts for 32% of the newly activated synapses at 24 hr, whereas newly formed varicosities account for 68%. |
PubMedSearch : Kim_2003_Neuron_40_151 |
PubMedID: 14527440 |
Kim JH, Udo H, Li HL, Youn TY, Chen M, Kandel ER, Bailey CH (2003)
Presynaptic activation of silent synapses and growth of new synapses contribute to intermediate and long-term facilitation in Aplysia
Neuron
40 :151
Kim JH, Udo H, Li HL, Youn TY, Chen M, Kandel ER, Bailey CH (2003)
Neuron
40 :151