Title : Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic Burkholderia sp. IS-01 - Kim_2009_J.Microbiol_47_651 |
Author(s) : Kim do Y , Park DS , Kwon SB , Chung MG , Bae KS , Park HY , Rhee YH |
Ref : J Microbiol , 47 :651 , 2009 |
Abstract :
Burkholderia sp. IS-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)] copolyesters with a high molar fraction of 3HV was isolated from the gut of the adult longicorn beetle, Moechotypa diphysis. The strain IS-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3HB-co-86.5 mol% 3HV) copolyester when cultivated on a mixture of gluconate (20 g/L) and levulinic acid (12.5 g/L). In this case, the content of the copolyester in the cells was approximately 60.0%. The compositions of the copolyesters were easily regulated by altering the molar ratio of gluconate and levulinic acid in the medium. The organism was found to possess a class I PHA synthase (PhaC) gene (1,881 bp) that encodes a protein with a deduced molecular mass of 68,538 Da that consists of 626 amino acids. The PhaC of this organism was most similar to that of B. cenocepacia PC184 (92% similarity). |
PubMedSearch : Kim_2009_J.Microbiol_47_651 |
PubMedID: 19851740 |
Kim do Y, Park DS, Kwon SB, Chung MG, Bae KS, Park HY, Rhee YH (2009)
Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic Burkholderia sp. IS-01
J Microbiol
47 :651
Kim do Y, Park DS, Kwon SB, Chung MG, Bae KS, Park HY, Rhee YH (2009)
J Microbiol
47 :651