Title : Production of a novel cold-active lipase from Pichia lynferdii Y-7723 - Kim_2010_J.Agric.Food.Chem_58_1322 |
Author(s) : Kim HR , Kim IH , Hou CT , Kwon KI , Shin BS |
Ref : Journal of Agricultural and Food Chemistry , 58 :1322 , 2010 |
Abstract :
Lipase (triacylglycerol acylhydrolases, E.C. 3.1.1.3) is one of the most important enzymes applied to a broad range of industrial application fields. Especially, lipases with abnormal functionality such as thermostability and alkaline, acidic, and cold activities gain special attention because of their applicability in the restricted reaction conditions. In this study, 16 yeast strains prescreened for lipase induction were investigated for their actual lipase production, and we found a novel cold-active lipase produced from Pichia lynferdii Y-7723. The activity of lipase Y-7723 was retained by 74 and 70% at 20 and 10 degrees C, respectively, as compared to the maximum value at 35 degrees C. On the basis of an optimization study, the optimal lipase productivity was obtained at 96 h of incubation with 3% oil substrate in a medium composed of sucrose as a carbon source at pH 7.0. Among carbon sources tested, sucrose showed almost twice as high of a lipase production (184%) as the control, while the cell growth was similar (105%). Yeast extract and ammonium salts were effective as individual nitrogen sources for lipase production. This study demonstrated that the cold activity of lipase Y-7723 at 10 degrees C was highest among the cold-active lipases reported so far. |
PubMedSearch : Kim_2010_J.Agric.Food.Chem_58_1322 |
PubMedID: 20000406 |
Kim HR, Kim IH, Hou CT, Kwon KI, Shin BS (2010)
Production of a novel cold-active lipase from Pichia lynferdii Y-7723
Journal of Agricultural and Food Chemistry
58 :1322
Kim HR, Kim IH, Hou CT, Kwon KI, Shin BS (2010)
Journal of Agricultural and Food Chemistry
58 :1322