Title : Lipase-Catalyzed Production of 6-O-cinnamoyl-sorbitol from D-sorbitol and Cinnamic Acid Esters - Kim_2015_Appl.Biochem.Biotechnol_176_244 |
Author(s) : Kim JH , Bhatia SK , Yoo D , Seo HM , Yi DH , Kim HJ , Lee JH , Choi KY , Kim KJ , Lee YK , Yang YH |
Ref : Appl Biochem Biotechnol , 176 :244 , 2015 |
Abstract :
To overcome the poor properties of solubility and stability of cinnamic acid, cinnamate derivatives with sugar alcohols were produced using the immobilized Candida antarctica lipase with vinyl cinnamate and D-sorbitol as substrate at 45 degrees C. Immobilized C. antarctica lipase was found to synthesize 6-O-cinnamoyl-sorbitol and confirmed by HPLC and (1)H-NMR and had a preference for vinyl cinnamate over other esters such as allyl-, ethyl-, and isobutyl cinnamate as co-substrate with D-sorbitol. Contrary to D-sorbitol, vinyl cinnamate, and cinnamic acid, the final product 6-O-cinnamoyl-sorbitol was found to have radical scavenging activity. This would be the first report on the biosynthesis of 6-O-cinnamoyl-sorbitol with immobilized enzyme from C. antarctica. |
PubMedSearch : Kim_2015_Appl.Biochem.Biotechnol_176_244 |
PubMedID: 25809993 |
Substrate | Sorbitol Isobutyl-cinnamate |
Kim JH, Bhatia SK, Yoo D, Seo HM, Yi DH, Kim HJ, Lee JH, Choi KY, Kim KJ, Lee YK, Yang YH (2015)
Lipase-Catalyzed Production of 6-O-cinnamoyl-sorbitol from D-sorbitol and Cinnamic Acid Esters
Appl Biochem Biotechnol
176 :244
Kim JH, Bhatia SK, Yoo D, Seo HM, Yi DH, Kim HJ, Lee JH, Choi KY, Kim KJ, Lee YK, Yang YH (2015)
Appl Biochem Biotechnol
176 :244