Ko_2009_Embo.J_28_3244

Reference

Title : Neuroligin-1 performs neurexin-dependent and neurexin-independent functions in synapse validation - Ko_2009_Embo.J_28_3244
Author(s) : Ko J , Zhang C , Arac D , Boucard AA , Brunger AT , Sudhof TC
Ref : EMBO Journal , 28 :3244 , 2009
Abstract :

Postsynaptic neuroligins are thought to perform essential functions in synapse validation and synaptic transmission by binding to, and dimerizing, presynaptic alpha- and beta-neurexins. To test this hypothesis, we examined the functional effects of neuroligin-1 mutations that impair only alpha-neurexin binding, block both alpha- and beta-neurexin binding, or abolish neuroligin-1 dimerization. Abolishing alpha-neurexin binding abrogated neuroligin-induced generation of neuronal synapses onto transfected non-neuronal cells in the so-called artificial synapse-formation assay, even though beta-neurexin binding was retained. Thus, in this assay, neuroligin-1 induces apparent synapse formation by binding to presynaptic alpha-neurexins. In transfected neurons, however, neither alpha- nor beta-neurexin binding was essential for the ability of postsynaptic neuroligin-1 to dramatically increase synapse density, suggesting a neurexin-independent mechanism of synapse formation. Moreover, neuroligin-1 dimerization was not required for either the non-neuronal or the neuronal synapse-formation assay. Nevertheless, both alpha-neurexin binding and neuroligin-1 dimerization were essential for the increase in apparent synapse size that is induced by neuroligin-1 in transfected neurons. Thus, neuroligin-1 performs diverse synaptic functions by mechanisms that include as essential components of alpha-neurexin binding and neuroligin dimerization, but extend beyond these activities.

PubMedSearch : Ko_2009_Embo.J_28_3244
PubMedID: 19730411
Gene_locus related to this paper: ratno-1neur

Related information

Mutation D242N\/Q243A\/K246A_ratno-1neur    Q256A\/R259A_ratno-1neur    Q328A\/Y332A_ratno-1neur    R271A\/Y372A\/H373A_ratno-1neur    L399A\/N400A\/D402N_ratno-1neur    D421N\/D423N\/D424N_ratno-1neur    D440N\/N441A\/D424N_ratno-1neur    N468A\/E470A\/R472A\/K474A_ratno-1neur    E542Q\/L543A\/F544A_ratno-1neur    F582A\/K586A\/R589A_ratno-1neur    E128Q\/V129A_ratno-1neur    E72Q\/I73A_ratno-1neur    D140N_ratno-1neur    H294A\/E297A_ratno-1neur    L302A\/S321A\/S322A_ratno-1neur    Y509A\/H511A\/Q513A_ratno-1neur    K306A\/L399A\/N400A\/D402N_ratno-1neur    E297A\/K306A\/L399A\/N400A\/D402N_ratno-1neur    L273A\/L399A\/N400A\/D402N_ratno-1neur    L399A\/N400A\/D402N\/K601A\/D602A_ratno-1neur    Q395A\/E397A\/L399A\/N400A\/D402N_ratno-1neur    L399A\/N400A\/D402N\/D571A\/Q574A_ratno-1neur    L399A\/N400A\/D402N\/F499A_ratno-1neur    T111A\/Q112A_ratno-1neur    E92A\/H93A_ratno-1neur    F458A\/W463A_ratno-1neur    Q147A\/D148A_ratno-1neur    R63A\/K66A_ratno-1neur    S102A\/S105A_ratno-1neur    E72Q\/I73A\/F582A\/K586A\/R589A_ratno-1neur    D421N\/D423N\/D424N\/Y509A\/H511A\/Q513A_ratno-1neur    Q328A\/Y332A\/D440N\/N441A\/Y445A_ratno-1neur    F458A\/M459A\/W463A_ratno-1neur    F458A\/M459A\/W463A\/L629A\/L633A_ratno-1neur    E297A\/K306A\/L399A\/N400A\/D402N\/F458A\/M459A\/W463A_ratno-1neur    A777X_ratno-1neur
Gene_locus D242N\/Q243A\/K246A_ratno-1neur    Q256A\/R259A_ratno-1neur    Q328A\/Y332A_ratno-1neur    R271A\/Y372A\/H373A_ratno-1neur    L399A\/N400A\/D402N_ratno-1neur    D421N\/D423N\/D424N_ratno-1neur    D440N\/N441A\/D424N_ratno-1neur    N468A\/E470A\/R472A\/K474A_ratno-1neur    E542Q\/L543A\/F544A_ratno-1neur    F582A\/K586A\/R589A_ratno-1neur    E128Q\/V129A_ratno-1neur    E72Q\/I73A_ratno-1neur    D140N_ratno-1neur    H294A\/E297A_ratno-1neur    L302A\/S321A\/S322A_ratno-1neur    Y509A\/H511A\/Q513A_ratno-1neur    K306A\/L399A\/N400A\/D402N_ratno-1neur    E297A\/K306A\/L399A\/N400A\/D402N_ratno-1neur    L273A\/L399A\/N400A\/D402N_ratno-1neur    L399A\/N400A\/D402N\/K601A\/D602A_ratno-1neur    Q395A\/E397A\/L399A\/N400A\/D402N_ratno-1neur    L399A\/N400A\/D402N\/D571A\/Q574A_ratno-1neur    L399A\/N400A\/D402N\/F499A_ratno-1neur    T111A\/Q112A_ratno-1neur    E92A\/H93A_ratno-1neur    F458A\/W463A_ratno-1neur    Q147A\/D148A_ratno-1neur    R63A\/K66A_ratno-1neur    S102A\/S105A_ratno-1neur    E72Q\/I73A\/F582A\/K586A\/R589A_ratno-1neur    D421N\/D423N\/D424N\/Y509A\/H511A\/Q513A_ratno-1neur    Q328A\/Y332A\/D440N\/N441A\/Y445A_ratno-1neur    F458A\/M459A\/W463A_ratno-1neur    F458A\/M459A\/W463A\/L629A\/L633A_ratno-1neur    E297A\/K306A\/L399A\/N400A\/D402N\/F458A\/M459A\/W463A_ratno-1neur    A777X_ratno-1neur    ratno-1neur

Citations formats

Ko J, Zhang C, Arac D, Boucard AA, Brunger AT, Sudhof TC (2009)
Neuroligin-1 performs neurexin-dependent and neurexin-independent functions in synapse validation
EMBO Journal 28 :3244

Ko J, Zhang C, Arac D, Boucard AA, Brunger AT, Sudhof TC (2009)
EMBO Journal 28 :3244