| Title : Simultaneous display of bacterial and fungal lipases on the cell surface of Bacillus subtilis - Kobayashi_2002_J.Biosci.Bioeng_93_15 |
| Author(s) : Kobayashi G , Fujii K , Serizawa M , Yamamoto H , Sekiguchi J |
| Ref : J Biosci Bioeng , 93 :15 , 2002 |
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Abstract :
A small cell wall-binding domain (CWB(c)) of the Bacillus subtilis peptidoglycan hydrolase CwlC fused to B. subtilis lipase B was able to be localized on the cell wall of B. subtilis. With the aim of developing an efficient lipid-hydrolyzing bacterium with two different lipases on the cell surface, plasmids possessing genes for LipB-CWB(c) and CWB(b)-CutL (the cell wall-binding domain of CwlB fused to the Aspergillus oryzae lipolytic enzyme CutL) were constructed. B. subtilis harboring these plasmids accumulated considerable amounts of both lipases on the cell surface. |
| PubMedSearch : Kobayashi_2002_J.Biosci.Bioeng_93_15 |
| PubMedID: 16233158 |
Kobayashi G, Fujii K, Serizawa M, Yamamoto H, Sekiguchi J (2002)
Simultaneous display of bacterial and fungal lipases on the cell surface of Bacillus subtilis
J Biosci Bioeng
93 :15
Kobayashi G, Fujii K, Serizawa M, Yamamoto H, Sekiguchi J (2002)
J Biosci Bioeng
93 :15