Title : Sequence analysis of the 2,4-dichlorophenol hydroxylase gene tfdB and 3,5-dichlorocatechol 1,2-dioxygenase gene tfdC of 2,4-dichlorophenoxyacetic acid degrading plasmid pEST4011 - Koiv_1996_Gene_174_293 |
Author(s) : Koiv V , Marits R , Heinaru A |
Ref : Gene , 174 :293 , 1996 |
Abstract :
Chlorocatechol 1,2-dioxygenase (CC12O) and 1,2-dichlorophenol hydroxylase (DCPH) encoding genes tfdC and tfdB are located on a 4.2-kb DNA fragment cloned from the 2,4-dichlorophenoxyacetic acid (2,4D) degrading plasmid pEST4011. The nucleotide sequences of tfdC and tfdB were determined. The DCPH is coded by a 1758-bp gene and CC12O is coded by a 762-bp gene. The deduced M(r) of these proteins are 64.09 kDa and 28.2 kDa, respectively. Expression analysis of tfdB and tfdC in Escherichia coli suggested that these genes form one operon, tfdCB. |
PubMedSearch : Koiv_1996_Gene_174_293 |
PubMedID: 8890750 |
Gene_locus related to this paper: psepu-q7m180 , varpa-TFDE |
Gene_locus | psepu-q7m180 varpa-TFDE |
Koiv V, Marits R, Heinaru A (1996)
Sequence analysis of the 2,4-dichlorophenol hydroxylase gene tfdB and 3,5-dichlorocatechol 1,2-dioxygenase gene tfdC of 2,4-dichlorophenoxyacetic acid degrading plasmid pEST4011
Gene
174 :293
Koiv V, Marits R, Heinaru A (1996)
Gene
174 :293