Kolesnikov_2022_Photochem.Photobiol__

Modern progress in photopharmocology calls for new generation of compounds joining bioactivity, photoswitchable properties and high selectivity of response to light wavelength. Introduced here phosphonate-fullerene hybrids are the first representatives of such compounds. Phosphonate-fullerene hybrids were synthesized on a base of fullerene C(60) and organophosphates with function of photoswitchable cholinesterase activity - phosphorylated thiazolotriazole and aminomalonate compounds, and studied with FTIR, UV-VIS spectroscopy, IPC-micro neurotoxin amperometric analysis. As a result of spectroscopic and bioactivity characterization it was demonstrated not only butyrylcholinesterase (BuChE) inhibition increase of phosphonate-fullerene hybrids compared to pure phosphonates, but also pronounced response of inhibition degree to laser irradiation of hybrids. It was found opposite behavior of hybrids as a result of laser irradiation - BuChE inhibition drop-off for thiazolotriazole-fullerene, and pronounced growth for aminomalonate-fullerene. The other remarkable peculiarity of presented phosphonate-fullerene hybrids is high selectivity of inhibition change degree to laser wavelength (266 nm or 325 nm).