Konno_2023_Sci.Adv_9_eadf7084

Reference

Title : Glyoxal fixation: An approach to solve immunohistochemical problem in neuroscience research - Konno_2023_Sci.Adv_9_eadf7084
Author(s) : Konno K , Yamasaki M , Miyazaki T , Watanabe M
Ref : Sci Adv , 9 :eadf7084 , 2023
Abstract : The gold-standard fixative for immunohistochemistry is 4% formaldehyde; however, it limits antibody access to target molecules that are buried within specialized neuronal components, such as ionotropic receptors at the postsynapse and voltage-gated ion channels at the axon initial segment, often requiring additional antigen-exposing techniques to detect their authentic signals. To solve this problem, we used glyoxal, a two-carbon atom di-aldehyde. We found that glyoxal fixation greatly improved antibody penetration and immunoreactivity, uncovering signals for buried molecules by conventional immunohistochemical procedures at light and electron microscopic levels. It also enhanced immunosignals of most other molecules, which are known to be detectable in formaldehyde-fixed sections. Furthermore, we unearthed several specific primary antibodies that were once judged to be unusable in formaldehyde-fixed tissues, allowing us to successfully localize so far controversial synaptic adhesion molecule Neuroligin 1. Thus, glyoxal is a highly effective fixative for immunostaining, and a side-by-side comparison of glyoxal and formaldehyde fixation is recommended for routine immunostaining in neuroscience research.
ESTHER : Konno_2023_Sci.Adv_9_eadf7084
PubMedSearch : Konno_2023_Sci.Adv_9_eadf7084
PubMedID: 37450597

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Konno K, Yamasaki M, Miyazaki T, Watanabe M (2023)
Glyoxal fixation: An approach to solve immunohistochemical problem in neuroscience research
Sci Adv 9 :eadf7084

Konno K, Yamasaki M, Miyazaki T, Watanabe M (2023)
Sci Adv 9 :eadf7084