| Title : Specific and sensitive plate assay for bacterial lipases - Kouker_1987_Appl.Environ.Microbiol_53_211 |
| Author(s) : Kouker G , Jaeger KE |
| Ref : Applied Environmental Microbiology , 53 :211 , 1987 |
|
Abstract :
A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat. |
| PubMedSearch : Kouker_1987_Appl.Environ.Microbiol_53_211 |
| PubMedID: 3103532 |
Kouker G, Jaeger KE (1987)
Specific and sensitive plate assay for bacterial lipases
Applied Environmental Microbiology
53 :211
Kouker G, Jaeger KE (1987)
Applied Environmental Microbiology
53 :211