Krzeslak_2012_FEMS.Microbiol.Lett_329_146

Reference

Title : Lipase A gene transcription in Pseudomonas alcaligenes is under control of RNA polymerase sigma54 and response regulator LipR - Krzeslak_2012_FEMS.Microbiol.Lett_329_146
Author(s) : Krzeslak J , Papaioannou E , van Merkerk R , Paal KA , Bischoff R , Cool RH , Quax WJ
Ref : FEMS Microbiology Letters , 329 :146 , 2012
Abstract :

Initial analysis has shown that the transcription of the Pseudomonas alcaligenes lipA gene, which encodes an extracellular lipase, is governed by the LipQR two-component system consisting of sensor kinase LipQ and DNA-binding regulator LipR. This study further analyzes lipA gene expression and demonstrates that the RNA polymerase sigma54 is involved in the transcription. Purified LipR has an ATPase activity that is stimulated by the presence of lipA promoter DNA. Surface plasmon resonance measurements with purified and in vitro phosphorylated LipR reveal that phosphorylation of LipR is required for specific binding to the upstream activating sequence of the lipA promoter. Furthermore, mass spectrometric analysis combined with mutagenesis demonstrates that Asp52 is the phosphorylated aspartate. This analysis exposes LipR as a prominent member of the growing family of bacterial enhancer-binding proteins.

PubMedSearch : Krzeslak_2012_FEMS.Microbiol.Lett_329_146
PubMedID: 22309406

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Citations formats

Krzeslak J, Papaioannou E, van Merkerk R, Paal KA, Bischoff R, Cool RH, Quax WJ (2012)
Lipase A gene transcription in Pseudomonas alcaligenes is under control of RNA polymerase sigma54 and response regulator LipR
FEMS Microbiology Letters 329 :146

Krzeslak J, Papaioannou E, van Merkerk R, Paal KA, Bischoff R, Cool RH, Quax WJ (2012)
FEMS Microbiology Letters 329 :146