| Title : Cloning and characterization of a bifunctional leukotriene A(4) hydrolase from Saccharomyces cerevisiae - Kull_1999_J.Biol.Chem_274_34683 |
| Author(s) : Kull F , Ohlson E , Haeggstrom JZ |
| Ref : Journal of Biological Chemistry , 274 :34683 , 1999 |
| Abstract : In mammals, leukotriene A(4) hydrolase is a bifunctional zinc metalloenzyme that catalyzes hydrolysis of leukotriene A(4) into the proinflammatory leukotriene B(4) and also possesses an arginyl aminopeptidase activity. We have cloned, expressed, and characterized a protein from Saccharomyces cerevisiae that is 42% identical to human leukotriene A(4) hydrolase. The purified protein is an anion-activated leucyl aminopeptidase, as assessed by p-nitroanilide substrates, and does not hydrolyze leukotriene A(4) into detectable amounts of leukotriene B(4). However, the S. cerevisiae enzyme can utilize leukotriene A(4) as substrate to produce a compound identified as 5S,6S-dihydroxy-7,9-trans-11, 14-cis-eicosatetraenoic acid. Both catalytic activities are inhibited by 3-(4-benzyloxyphenyl)-2-(R)-amino-1-propanethiol (thioamine), a competitive inhibitor of human leukotriene A(4) hydrolase. Furthermore, the peptide cleaving activity of the S. cerevisiae enzyme was stimulated approximately 10-fold by leukotriene A(4) with kinetics indicating the presence of a lipid binding site. Nonenzymatic hydrolysis products of leukotriene A(4), leukotriene B(4), arachidonic acid, or phosphatidylcholine were without effect. Moreover, leukotriene A(4) could displace the inhibitor thioamine and restore maximal aminopeptidase activity, indicating that the leukotriene A(4) binding site is located at the active center of the enzyme. Hence, the S. cerevisiae leukotriene A(4) hydrolase is a bifunctional enzyme and appears to be an early ancestor to mammalian leukotriene A(4) hydrolases. |
| ESTHER : Kull_1999_J.Biol.Chem_274_34683 |
| PubMedSearch : Kull_1999_J.Biol.Chem_274_34683 |
| PubMedID: 10574934 |
Kull F, Ohlson E, Haeggstrom JZ (1999)
Cloning and characterization of a bifunctional leukotriene A(4) hydrolase from Saccharomyces cerevisiae
Journal of Biological Chemistry
274 :34683
Kull F, Ohlson E, Haeggstrom JZ (1999)
Journal of Biological Chemistry
274 :34683