| Title : Enantiomeric separation of pharmaceutically important drug intermediates using a Metagenomic lipase and optimization of its large scale production - Kumar_2017_Int.J.Biol.Macromol_95_995 |
| Author(s) : Kumar R , Banoth L , Banerjee UC , Kaur J |
| Ref : Int J Biol Macromol , 95 :995 , 2017 |
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Abstract :
In the present study, efficient enzymatic methods were developed using a recombinant metagenomic lipase (LipR1) for the synthesis of corresponding esters by the transesterification of five different pharmaceutically important secondary alcohols. The recombinant lipase (specific activity=87m6U/mg) showed maximum conversion in presence of ionic liquid with Naphthyl-ethanol (eeP=99%), Indanol and Methyl-4 pyridine methanol (eeS of 98% and 99%) respectively in 1h. Vinyl acetate was found as suitable acyl donor in transesterification reactions. It was interesting to observe that maximum eeP of 85% was observed in just 15min with 1-indanol. As this enzyme demonstrated pharmaceutical applications, attempts were made to scale up the enzyme production on a pilot scale in a 5litre bioreactor. Different physical parameters affecting enzyme production and biomass concentration such as agitation rate, aeration rate and inoculum concentration were evaluated. Maximum lipase activity of 8463U/ml was obtained at 7h of cultivation at 1 lpm, 300rpm and 1.5% inoculum. |
| PubMedSearch : Kumar_2017_Int.J.Biol.Macromol_95_995 |
| PubMedID: 27984150 |
Kumar R, Banoth L, Banerjee UC, Kaur J (2017)
Enantiomeric separation of pharmaceutically important drug intermediates using a Metagenomic lipase and optimization of its large scale production
Int J Biol Macromol
95 :995
Kumar R, Banoth L, Banerjee UC, Kaur J (2017)
Int J Biol Macromol
95 :995